RKIP regulates CCL5 expression to inhibit breast cancer invasion and metastasis by controlling macrophage infiltration
نویسندگان
چکیده
Cell lines and reagents: Matrigel was from BD Biosciences. The highly metastatic lung-tropic MDA-MB231 subline 4175 was kindly provided by Dr. J. Massague [1]. MCF10A, 4T1, and 4T07 cells were kindly provided by Dr. Fred Miller (Karmanos Cancer Institute, MI). BT20 and MCF7 were purchased from ATCC. 4T1, and 4T07 cells were cultured in Dulbecco’s modified Eagle’s medium with 5% FBS, 5% newborn calf serum and 1% pen-strep. BT20, MCF7, and MDA-MB-231_4175 cells were cultured in Dulbecco’s modified Eagle’s medium with 10% FBS. Cells were grown in a humidified tissue culture incubator at 37°C in 5% CO2.
منابع مشابه
Metastasis Suppressors Regulate the Tumor Microenvironment by Blocking Recruitment of Prometastatic Tumor-Associated Macrophages.
Triple-negative breast cancer (TNBC) patients have the highest risk of recurrence and metastasis. Because they cannot be treated with targeted therapies, and many do not respond to chemotherapy, they represent a clinically underserved group. TNBC is characterized by reduced expression of metastasis suppressors such as Raf kinase inhibitory protein (RKIP), which inhibits tumor invasiveness. Mech...
متن کاملMicroenvironment and Immunology Metastasis Suppressors Regulate the Tumor Microenvironment by Blocking Recruitment of Prometastatic Tumor-Associated Macrophages
Triple-negative breast cancer (TNBC) patients have the highest risk of recurrence and metastasis. Because they cannot be treated with targeted therapies, and many do not respond to chemotherapy, they represent a clinically underserved group. TNBC is characterized by reduced expression of metastasis suppressors such as Raf kinase inhibitory protein (RKIP), which inhibits tumor invasiveness. Mech...
متن کاملCorrection: RKIP regulates CCL5 expression to inhibit breast cancer invasion and metastasis by controlling macrophage infiltration
Present: Due to an error made by the authors while submitting a revision, Dr. Tuan Zea Tan was omitted from the list of authors.Corrected: Correct author list can be found below. Authors sincerely apologize for this oversight. Ila Datar1, Xiaoliang Qiu1, Hong Zhi Ma1, Miranda Yeung1, Shweta Aras1, Ivana de la Serna1, Fahd Al-Mulla2, Tuan Zea Tan3, Jean Paul Thiery3, Robert Trumbly1, Xuan Fan4, ...
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Raf Kinase Inhibitory Protein or RKIP was initially identified as a Raf-1 binding protein using the yeast 2-hybrid screen. RKIP inhibits the activation phosphorylation of MEK by Raf-1 by competitively inhibiting the binding of MEK to Raf-1 and thus exerting an inhibitory effect on the Raf-MEK-Erk pathway. RKIP has been identified as a metastasis suppressor gene. Expression of RKIP is low in can...
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